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      | ֙C | EN | | ҂ķ
      ʳƷW(wng)̖
      ǰλ: » z򞼼g(sh) » ʳƷz򞼼g(sh) » x »

      Һ|(zh)“(lin)MApɽQN}

      Ŵw  sСw l(f)ڣ2022-01-19
      ʾ|(zh)Vкܸߵ`ȺͷֱڶԺͶкЃ(yu)ԬF(xin)|(zh)VČԽԽ|(zh)VɫVfˌh(hun)

      |(zh)Vкܸߵ`ȺͷֱڶԺͶкЃ(yu)ԬF(xin)|(zh)VČԽԽ|(zh)VɫVfˌh(hun)Ҫ;SoҲӷÿƷƲͬϵеľSoҪҲͬ|(zh)VxЩľSoľͿY(ji)|(zh)VxճҪľSomô󲿷ֵҺ|(zh)“(lin)Ãx

       

      Һ|(zh)“(lin)(HPLC-MS)ֽҺɫV-|(zh)V“(lin)üg(sh)ҺɫVxϵy(tng)|(zh)Vzyϵy(tng)Ʒ|(zh)Vֺxxӻ(jng)|(zh)V|(zh)xƬ|(zh)(sh)_(jng)zyõ|(zh)VDҺ|(zh)“(lin)wF(xin)ɫV|(zh)V(yu)ݵĻaɫVsƷĸ߷xcMSиx`ȼ܉ṩ|(zh)cY(ji)(gu)Ϣă(yu)cY(ji)ˎʳƷͭh(hun)SI(lng)õˏVđá

       

      һ|(zh)Vxh(hun)


       

      1.|(zh)Vxh(hun)Ҫ:

      ܇oʎԴ늴ŸБb;

      ԴҪӵؽ;

      ҜҪ1528;

      Ҫ20%80%

      Ҋʹ^Ҫ؄eע҃(ni)ضȺ͝ȵĿһ]wйܵwЕrg|(zh)Vh(hun)Ҫⲿh(hun)ֱӰ|(zh)SĜʴ_|(zh)VxÃɼY(ji)(gu)ǰCе󼉞Ӝu݆ùrǰǻ(ni)ĉׂ͎(sh)ɺ󼉱ý艺

       

      Һ|(zh)ԭc



       

      1LC-MSԭ

      Һ|(zh)“(lin)ҺɫVxϵy(tng)|(zh)Vzyϵy(tng)Ʒ|(zh)Vֺxxӻ (jng)|(zh)V|(zh)xƬ|(zh)ȷ_(jng)zyõ|(zh)VD

      wF(xin)ɫV|(zh)V(yu)ݵĻaɫVsƷĸ߷xcMSиx`ȼ܉ṩ|(zh)cY(ji)(gu)Ϣă(yu)cY(ji)ˎʳƷͭh(hun)SI(lng)õˏVđ

       

      2õăɴϵy(tng)

      һNǏ|(zh)VxԴǶȁ턝늇FxԴ(ESI)≺WxԴ(APCI)≺xԴ(APPI)ͻ|(zh)oxԴ(MALDI)

      һNǏ|(zh)V|(zh)Ƕȁ턝֣ĘOUxwЕrg(TOF)͸~׃Q|(zh)VESIAPCIAPPINxԴcĘOUx|(zh)V“(lin)ĿǰVĎ׷NҺ|(zh)“(lin)Ãx

       

      3Ҫc

      LC-MS˿Է|(zh)“(lin)ܷďOԡy]l(f)᲻(wn)ԵĻ֮⣬߀Žׂă(yu)c

      VMS׺ԙzyеĻ^׵ؽQ˷᲻(wn)y};

      xʹɫVϛ]ȫx_ͨ^MSx|(zh)ɫVDҲܷքeoԵɫVDMжԶ;

      ԷY(ji)ɿͬroÿһMֵķSĽY(ji)(gu)Ϣ;

      zy޵MS߂`ȣͨ^xx(SIM)zyʽzy߀һ(sh);

      rgLC-MSʹõҺɫVխs˷rg˷xЧ;

      Ԅӻ̶ȸLC-MSи߶ȵԄӻ

       




       

      1_C

      |(zh)Vx_C__Cеãٴ_|(zh)VxԴն__Ӝu݆ն_ſM{(dio)CУһҪ12Сr_ÿ_CҪУʹ|(zh)Vx

       

      2Ʒyԇ^̣

      2.1.cҺɫV“(lin)ÕrҪ<0.45μmĤ^VҪ^(q)eЙCĤˮĤƷҲͬҪ^V>10000rpmD(zhun)xȥws|(zh)

      2.2಻y]l(f)}}}ҺೣõTFAxxҲhʹԄ|(zh)V푑ܸߣESIԴйܺߵϴϴÁƷxɫVεxӌԇҲác|(zh)V“(lin)ÕrhʹõǼᡢ@@Ͱˮȡ

      2.3(j)xõxԴ{(dio)Һ෽ESIԴһ0.3~0.6mL/min,Ҏ(gu)HPLCҎ(gu)5μm4.6×250mmһٶ1mL/minԲķʽ{(dio)M|(zh)VͬrҪ(j)M|(zh)V͘Ʒ|(zh){(dio)FضȺF

      2.4ƷyԇY(ji)ҪϴMӹ·ϴͣãxԴضȽͺxCB(ti)

       

      ģҺ|(zh)ճھSo



       

      1ʹõķ(wn)ԴUPSCxԴ늉(wn)m(x)(؄eͣ늕rxӰ)

      2ÿîҺ_ϴϵy(tng)坍F_y(ESIԴÿһ;APCIԴÿһ)Cëܝ

      3ٲҪ^ߣESIxԴҪ^0.5ml/minAPCIҪ^1.0ml/min

      4CеõıҪڸQһQһΣͿKҪǰQQ͵ĕrҪȫٸQµıͣͬƷƵıͲܻһ㌍ҵ|(zh)VxLٵĕrMдľSo

      5ƺØƷ\еĭh(hun)ضȺ͝

      6ꮅҪϴMMy^ɫVxԴҪü״/ˮ_ϴ

      7ϴƷFP(gun)]yȡ˜ƷFü״ˮ(454510)Һ•ϴ10犣Ȼڷքeóˮͼ״Һ•ϴ10ɺٰbx

      8ҪF(xin)ìF(xin)òҪ^ҹһҪr
          9ڸQӱ

       
      ݋songjiajie2010

       
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