1|VĿԭ
1Mϵy(tng)cxԴ]Bӻ©Һ
2ͨy©Һ
3F]_
4F늉]
5xMxͨ
6Fëܶ
2θ(j)ƷxxԴ
𣺿ɸ(j)ĴСOAPCImСOСĻESImϷķ^ҪһOԡһȿ]ESIOԌ̫С뵽APCI
3ȶ߀ݶϴÓx
䌍ֻһɂǵȶϴÓٶȿҲԽԽãeڷƷr]|Чӿ2-3^ݶϴÓmϷYͬ|绯cxa(chn)һͬbĕrպԪһͬyܶϳɻWķõҲһͨõݶϴÓһ㶨֘ƷһfڽMɺεĘƷԲõȶϴÓЩsĘƷxͨҪMݶϴÓ
4|Һ|ϵy(tng)
𣺳˲õķxֶβͬҺࣩҪą^(q)eϵy(tng)xʽ|ϵy(tng)^ֻҪһСęCеúһӜu݆þͿҺ|ęCеҪȚ|ҪɂӜu݆|xʽx(EI)ͻWx(CI)Һ|xʽ늇Fx(ESI)≺Wx(APCI)ʹ≺x(APPI)
5APCIESIIJͬc
1xӮa(chn)ķʽͬAPCI늕xӻxӻESIxl(f)Һxӻ
2ܱĻͲͬAPCImOСӻҾһē]l(f)ESI OԻ
3ٲͬESIһ^Сs0.0010.25 mL/minAPCI ^s0.22 mL/min
4늺APCIһϵж늺xԲmϷESI һϵж늺xӣemڵ
6CIDCAD^(q)e
CIDײTxͨսӿ̎{(ji)늉l(f)CIDF(xin)һȥ܄늉Ҳa(chn)Ƭx@һ|VԭCADײx|VrxĸxMQ2ײa(chn)x@^̷Q CADAPI3200VxCIDָQ0TײĚwCADָQ3TײwҲf@CID&CADָ⡣
7l(f)ԓʹÆ
𣺵l(f)ĹԭǷx՚늽ĤؓOȰl(f)“Ե”՚еԚwO߀ԭ՚^늽غֻʣµͶԚwʇȰl(f)ļȴ“”ļȺͿ՚ЧֽL늽늄ݵďPϵ@NxҲQ˵ļȲĺܸߡ늽|þˮČʹ늻WMl(f)|VӰһcԣǰl(f)ȵ_PԴr늾W(wng)늉һĸɔ_sCĆӺֹͣҲ
8(lin)|Vζ
𣺴(lin)|Vr,Ժa(chn)Ƭ壨xӣ@һxĸxײҮa(chn)ƬMRM`ȕSIMúܶIJEǣһܶȵĘ˜ƷҺ1-10 ug/mL{Cһ|Vlҵĸxӵ|VlȻĸxMд(yu)ײõԵxԓ|Vlԓĸx->xӌMж
9|ƫôk
|V|(sh)ƫfăxԓУˣһ3¾ҪУһΙCһÿSҶSCУҺ
10θQCеͣ
һ3µQһαͬrͣCxMһϴQ͵ĕr_y5-10ÃȳP]yͬrP]Դ_µıŷyTŵf͡ѽ(jng)KtȡSϴúŵע
11a(chn)bxӽӰ푣Collision Cell Cross Talkԭ
𣺶ͨ裨MRMrɂxӒͨƬxһӣ1-2λǰһxͨYbxӁӰһxͨĶɫVxȫtoӰ푣
ܵ
1xخxӣe÷(wn)ͬλȘ˕r
2xͨ跴֮gĕrgg100 ms→300 ms
3O~“oõxӒͨdummy MRM”
12ųɫVʧ}ѷʲô
\ɫVǷʧ}ķǵһڷl°bɫVrһοհɫVDȻ\кͿհ\ɫVDȡڿհ\Юa(chn)˺ܶtɫVܸ׃@dкҲژƷ GC-MSt͘OɫVĵʧxӣ DB/HP-15|/ɱm/z 20773281355 ȣ(sh)h(hun)
13VDʲôֻ܄
𣺿ԭ
1Mᘓp
2d̫
3Ʒ̫
4ƷMr
14|Vʲôԭ
|VĻ䌍Һzy͟ɹzyһߵԭȲⲿԭ
1x|V푑^ߣˮ^ĕr^Щ߀}^ĕrЩ
2zy`ԽߵĕrԓԽ|VȾ^ؕr϶^ߡxzyõþˣеxӾ͕һ潵|V`һ˻
3|VĻܶr߀xxӌPxxӒĕr͵ЩxĕrxӌȲҪx̫̫Щ
4༉|Vһ|V͵ͺܶ