（也稱基因工程、分子生物學(xué)技術(shù)或克隆）純化、處理和使用遺傳材料的技術(shù)總和。這些技術(shù)主要基于對(duì)從細(xì)菌和動(dòng)物細(xì)胞里純化出的酶的使用，使得DNA復(fù)制、轉(zhuǎn)化為RNA、轉(zhuǎn)譯為蛋白質(zhì)都可以在實(shí)驗(yàn)室完成。這些步驟包括在DNA特異位點(diǎn)切割，連接和切斷DNA片段和很多其它修飾直至對(duì)單個(gè)堿基對(duì)的處理。這些技術(shù)使得DNA的處理、在細(xì)菌和酵母體內(nèi)將這些基因通過(guò)表達(dá)轉(zhuǎn)化成蛋白質(zhì)、病毒生物工程、轉(zhuǎn)基因動(dòng)物和滅活屬等生物工程產(chǎn)物、基于DNA的法庭診斷測(cè)試的發(fā)展成為可能。

(Also known as genetic engineering, molecular biology technology or cloning.) The collection of techniques that allow the purification, manipulation and use of genetic material. These techniques are mostly based on the use of enzymes purified from bacteria and animal cells, that allow steps such as DNA copying (replication), conversion into RNA (transcription), and into protein (translation), to be recapitulated in the laboratory. These steps include specific cutting of DNA at defined sites, joining or splicing of DNA fragments, and numerous other modifications which allow manipulation down to the level of a single base pair. These techniques allow the manipulation of DNA; the conversion of these genes into protein by expression in biological vats such as bacteria, and yeast; the bioengineering of viruses; the production of engineered organisms such as transgenic animals and knockout mice; and the development of DNA-based forensic and diagnostic tests.