mȻPCRxһNӋ(j)xҪԭcӋ(j)ҪP(gun)Ҫ^һʧxPCRxҲҪڙzy;So(h)@هȻL(fng)صPCRxȞҪ
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S o(h) B(yng)
1.PCRxҪڙzyҕ䷽ʽһһ
2.PCR(yng)Ҫضc(sh)Hֲķ(yng)ضDzһµ(dng)zyl(f)F(xin)ƽضȲƫxO(sh)Üضȴ1-2r(sh)\(yn)ÜضmPCR(sh)H(yng)ضȲ
3.PCR(yng)^̵P(gun)I^̵ĕr(sh)gҪԽԽ(dng)PCRxĽ^̳^60s͑(yng)ԓzxϵy(tng)L(fng)PCRxҪ^ص(yng)Ļ҉mϵy(tng)(yng)zP(gun)䲿
4.һrܲÜضmxĜضȕr(sh)Ҫp״_{(dio)xӿƲҪr(sh)ҪՈI(y)ˆTÃxӾ·Ԕ(x)DM(jn)оSޡ
1.Ʒصϴ
ȴ_wӣȻ95%Ҵ10%ϴҺݘƷ5minȻϴȾĿףҺȡҺwʣҺw_PCRxO(sh)֜ضȞ50PCRʹ֮\(yn)Һw]l(f)ȥһ5-10min
2.wϴ
ڟɹⶨPCRx(dng)ПɹȾF(xin)@һȾǁԘƷؕr(sh)(dng)ȾEӰ푵wɾor(sh)ҪÉs՚ˮϴ|w棬_ƷصĿɃo·
3.xϴ
ϴxԳȥ҉m֬_(d)Чx]иgԵϴPCRxM(jn)ϴ
4.QU(xin)z
ȌPCRxP(gun)C(j)ȥ^_Դ߅ıU(xin)УQςõıU(xin)z^Ƿ֏(f)